Special Precautions/Comments:

N.B. Although blood is the preferred sample type; CSF (if querying CMV encephalitis) and urine (paediatric patients) are acceptable sample types.

Method:  Real-time polymerase chain reaction (PCR) for the detection of Cytomegalovirus DNA. Calibration: – EQA scheme: UKNEQAS IQC: –

Interpretation: Unless otherwise requested, blood samples will be tested using a multiplex PCR assay for the detection of Cytomegalovirus (CMV), Epstein-Barr Virus (EBV) and Adenovirus (AD). Results are reported as NOT detected or DETECTED by PCR. A negative result does NOT fully exclude infection.

For further information contact Department of Microbiology, Newcastle Laboratories, Freeman Hospital.

Additional Information:

Background information:  Cytomegalovirus (CMV) is a herpesvirus which can cause serious diseases in infants and adults. CMV can persist in the human body for several years and can cause recurrent infections or be transmitted to other individuals. CMV infections are very common; 60 to 85% of the population have been infected but most cases are asymptomatic. In immunocompromised patients (HIV positive or organ transplanted), CMV infections can be severe.

Detection of CMV DNA by PCR can aid the diagnosis and monitoring of immunocompromised patients. CMV PCR may also be used in the diagnosis of congenital CMV infection. If congenital CMV is suspected please send a urine sample 2-3 weeks after birth. If CMV infection is suspected in an immunocompetent patient, recommend requesting CMV IgM (CMVM) testing instead of CMV PCR.