Special Precautions/Comments:

N.B. Please provide a detailed travel history.

Method: Enzyme immunoassay (EIA) for the determination of Dengue IgG and IgM antibody levels. Real-time polymerase chain reaction (RT-PCR) for the detection of Dengue virus DNA. Calibration: – EQA scheme: – IQC: –

Interpretation: Results will be reported as DETECTED (positive) or NOT detected (negative). A negative PCR result does not exclude previous infection. Clinical comments will be provided based on patient history.

For further information contact UKHSA Porton Down, Rare and imported pathogens laboratory (RIPL).

Additional Information:

Background information: Dengue fever is a non-endemic to the UK and is caused by a viral infection spread by Aedes mosquitoes. Dengue fever should be considered for patients who have recently returned from endemic areas and are presenting with severe flu-like illness and fever, this pertains to large regions of South and South East Asia, South and Central America, Africa and the Caribbean. Dengue fever has also been noted in parts of Southern Europe during Spring-Summer months, including France, Italy and Spain.